KMID : 1100720190390020176
|
|
Annals of Laboratory Medicine 2019 Volume.39 No. 2 p.176 ~ p.182
|
|
Comparative Evaluation Between the RealStar Pneumocystis jirovecii PCR Kit and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR Kit for Detecting P. jirovecii in Non-HIV Immunocompromised Patients
|
|
Huh Hee-Jae
Lim Kyoung-Ree Ki Chang-Seok Huh Kyung-Min Shim Hyang-Jin Song Dong-Joon Kim Yae-Jean Chung Doo-Ryeon Lee Nam-Yong
|
|
Abstract
|
|
|
Background: Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii. We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR kit (InterLabService Ltd., Moscow, Russia).
Methods: We used 159 samples from the lower respiratory tract (112 bronchoalveolar lavage [BAL] fluid, 37 sputum, and 10 endotracheal aspirate [ETA] samples) of non-HIV immunocompromised patients. Nested PCR and sequencing were used to resolve discordant results. The performance of the two assays was evaluated according to clinical categories (clinical Pneumocystis pneumonia [PCP], possible PCP, or unlikely PCP) based on clinical and radiological observations.
Results: The positive and negative percent agreement values were 100% (95% confidence interval [CI], 85.4?100%) and 96.6% (95% CI, 90.9?98.9%), respectively, and kappa was 0.92 (95% CI, 0.84?0.99). P. jirovecii DNA load was significantly higher in the clinical PCP group than in the other groups (P<0.05). When stratified by sample type, the positive rate for BAL fluids from the clinical PCP group was 100% using either assay, whereas the positive rate for sputum/ETA samples was only 20%.
Conclusions: The two assays showed similar diagnostic performance and detected low P. jirovecii burden in BAL fluids. Both assays may be useful as routine methods for detecting P. jirovecii DNA in a clinical laboratory setting, though their results should be interpreted considering sample type.
|
|
KEYWORD
|
|
Pneumocystis jirovecii, Pneumocystis pneumonia, Real-time PCR, RealStar Pneumocystis jirovecii PCR, AmpliSens Pneumocystis jirovecii (carinii)-FRT PCR, Performance
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|